Is Upgrading From (23.1.a.1.28) To (23.4.A.0.546) Remove Root ?!

Hi
My Question Like In Title : Is Upgrading From (23.1.a.1.28) To (23.4.A.0.546) Remove Root ?! Yes Or No ?
If Yes I Have (23.1.a.1.28) With Root How Can I Upgrade To (23.4.A.0.546) With Root ?
Thanks Alot